Resources
The following resources will help you utilize the HuProt™ Human Proteome Microarray. Please return to this page for new information that will be added soon, including new applications, data analysis techniques, usage tips, links to selected research publications and other articles of interest.
-
USER GUIDE
HuProt™ Human Proteome Microarray v3.1 -
HuProt CONTENT
Multifunction web-based search utility -
HuProt FAQs
A comprehensive collection of topics relating to the handling, content, use and applications of the HuProt Human Proteome Microarray -
NEW
ImmuneProfiler
Poster: SITC 11/18Hulett, et al. “Pre-treatment IgG autoantibodies associated with favorable Ipilimumab outcomes in melanoma include testis antigens and proteins linked to neurodegeneration”
-
HuProt Poster: ASCO 6/17
Gowen, et al. “Predictive Biomarkers of Immunotherapy Toxicity in Metastatic Melanoma Patients” -
HuProt Poster
Hu, et al. “Proteins on HuProt™ Array Are Well Folded” -
HuProt lot-specific COAs and associated GENPIX .gal files
Files compatible with GenePix® scanners and essentially are a map of all the proteins contained on the HuProt microarray corresponding to its lot number. If you have any questions on data analysis of CDI’s HuProt arrays please contact Technical Support .
Products & Services Literature
CDI brochures - in PDF format - can be downloaded from our website. Simply click on an image. We are happy to send printed literature by mail too. Please email your request to [email protected] . Stay in touch by joining our mailing list for information on the latest products and services.
Events
-
SITC - Society For Immunotherapy of Cancer Annual Meeting 2018
November 7-11, 2018
Walter E. Washington Convention Center Washington, D.C.VISIT CDI at BOOTH 6
Proteomics and Molecular Medicine
November 26-28, 2018
Dublin, Ireland
13th World Biomarkers Congress
November 29-30, 2018
Dublin, Ireland
Protein & Proteomics European Congress
December 06-07, 2018
Amsterdam, Netherlands
Antibody Engineering & Therapeutics
December 9-13, 2018
San Diego, CA USA
Antibodies as Drugs: New Horizons in the Therapeutic Use of Engineered Antibodies
April 7—11, 2019
Breckenridge, CO USAHuProt™ Human Proteome Microarray Literature Citations
Lastwika KJ et al. (2018) Tumor-Derived Autoantibodies Identify Malignant Pulmonary Nodules. Am J Respir Crit Care Med Nov 13. doi: 10.1164/rccm.201804-0628OC. [Epub ahead of print][Abstract]
Gowans MF et al. (2018) Baseline antibody profiles predict toxicity in melanoma patients treated with immune checkpoint inhibitors. J Transl Med 16:82. [Full Text]
Venkataraman A et al. (2018) A toolbox of immunoprecipitation-grade monoclonal antibodies to human transcription factors. Nature Methods doi:10.1038/nmeth.4632. [Full Text]
Chung JM et al. (2018) Identification of the Thioredoxin-Like 2 Autoantibody as a Specific Biomarker for Triple-Negative Breast Cancer. J Breast Cancer 21:87-90. [Full Text]
Bremmer HD et al. (2018) ILF2 and ILF3 are autoantigens in canine systemic autoimmune disease. Sci Rep 8:4852. [PubMed] [Full Text]
Coll JM (2018) Herpesvirus Infection Induces both Specific and Heterologous Antiviral Antibodies in Carp. Front Immunol 9:39.
[PubMed] [Full Text]Guo G et al. (2018) The cytomegalovirus protein US31 induces inflammation through mono-macrophages in systemic lupus erythematosus by promoting NF-κB2 activation. Cell Death Disease 9:104. doi: 10.1038/s41419-017-0122-4. [PubMed] [Full Text]
Pan J et al. (2017) Identification of Serological Biomarkers for Early Diagnosisof Lung Cancer Using a Protein Array-Based Approach. Mol Cell Proteomics 16:2069-2078. [PubMed] [Full Text]
Huang Y and Zhu H (2017) Protein Array-based Approaches for Biomarker Discovery in Cancer. Genomics Proteomics Bioinformatics 15:73-81. [PubMed] [Full Text]
Bigley V et al. (2017) Biallelic interferon regulatory factor 8 mutation: A complex immunodeficiency syndrome with dendritic cell deficiency, monocytopenia, and immune dysregulation. J Allergy Clin Immunol doi.org/10.1016/j.jaci.2017.08.044 [PubMed] [Full Text]
Sterner E et al. (2017) Therapeutic Antibodies to Ganglioside GD2 Evolved from Highly Selective Germline Antibodies. Cell Rep 20:1681-1691. [PubMed] [Full Text]
Washburn N et al. (2017) High-resolution physicochemical characterization of different intravenous immunoglobulin products. PLoS One 12(7):e0181251. doi: 10.1371/journal.pone.0181251. [PubMed] [Full Text]
Xu Z et al. (2017) Systematic identification of the protein substrates of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase-T1/T2/T3 using a human proteome microarray. Proteomics 17(11):doi: 10.1002/pmic.201600485 [PubMed]
Yang Z et al. (2017) A Human Proteome Array Approach to Identifying Key Host Proteins Targeted by Toxoplasma Kinase ROP18. Mol Cell Proteomics 16(3):469-484 [Full Text]
Gupta S et al. (2017) Evaluation of autoantibody signatures in meningioma patients using human proteome arrays. Oncotarget Apr 10. doi: 10.18632/oncotarget.16997. [PubMed] [Full Text]
Chung BK et al. (2017) Phenotyping and auto-antibody production by liver-infiltrating B cells in primary sclerosing cholangitis and primary biliary cholangitis. J Autoimmun 77:45-54. [PubMed]
Cheng X et al. (2017) Proteomic identification of the oncoprotein STAT3 as a target of a novel Skp1 inhibitor. Oncotarget 8(2):2681-2693. [Full Text]
Gupta S et al. (2017) Serum Profiling for Identification of Autoantibody Signatures in Diseases Using Protein Microarrays. Methods Mol Biol 1619:303-315. [PubMed]
Cox E et al. (2017) Global Analysis of SUMO-Binding Proteins Identifies SUMOylation as a Key Regulator of the INO80 Chromatin Remodeling Complex. Mol Cell Proteomics 16, Mar 2. doi: 10.1074/mcp.M116.063719. [Full Text]
Barry G et al. (2017) The long non-coding RNA NEAT1 is responsive to neuronal activity and is associated with hyperexcitability states. Nature Scientific Reports 7, Article number: 40127 doi:10.1038/srep40127 [Full Text]
Shi L et al. (2016) Application of high-throughput protein array in clinical screening for tumor markers. Int J Clin Exp Med 9:8529-8535.
[Full Text]Iyama S et al. (2016) Drebrin: A new oncofetal biomarker associated with prognosis of lung adenocarcinoma. Lung Cancer 102:74-81. [PubMed]
Blackshaw S et al. (2016) The NIH Protein Capture Reagents Program (PCRP): a standardized protein affinity reagent toolbox. Nat Methods 13:805-806. [PubMed]
Cheng X et al. (2016) Proteomic identification of the oncoprotein STAT3 as a target of a novel Skp1 inhibitor. Oncotarget DOI: 10.18632/oncotarget.13153. [PubMed]
Hu CJ et al. (2016) Identification of Novel Biomarkers for Behcet Disease Diagnosis Using HuProt Array Approach. Mol Cell Proteomics 2016 Oct 24. pii: mcp.M. 116.061002. [PubMed]
Wang Y et al. (2016) A nuclease that mediates cell death induced by DNA damage and poly(ADP-ribose) polymerase-1. Science 354 2016 Oct 7 DOI: 10.1126/science.aad6872. [Link]
Ogishi M et al. (2016) Delineation of autoantibody repertoire through differential proteogenomics in hepatitis C virus-induced cryoglobulinemia. Sci Rep 6:29532. [PubMed, Full Text]
Li H et al. (2016) Penetrance of Congenital Heart Disease in a Mouse Model of Down Syndrome Depends on a Trisomic Potentiator of a Disomic Modifier. Genetics2016 Mar 30. pii: genetics.116.188045. [PubMed]
Yang L et al. (2016) Identification of serum biomarkers for gastric cancer diagnosis using a human proteome microarray. Mol Cell Proteomics 15 (2):614-23. doi: 10.1074/mcp.M115.051250. Epub 2015 Nov 23 [PubMed]
Syed P et al.(2016) Autoantibody Profiling of Glioma Serum Samples to Identify Biomarkers Using Human Proteome Arrays Sci Rep2015 Sep 15;5:13895. doi: 10.1038/srep13895. [PubMed]
Dart AE et al. (2015) PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion. J Cell Biol 211:863-879.
[PubMed] [Full Text]Ainscough JS et al. (2015) Interleukin-1β Processing Is Dependent on a Calcium-mediated Interaction with Calmodulin. J Biol Chem 290:31151-61. [PubMed] [Full Text]
Zhang K et al. (2015) The C9orf72 repeat expansion disrupts nucleocytoplasmic transport. Nature 525:56-61. [PubMed] [Full Text]
Zhang HN et al. (2015) Systematic identification of arsenic-binding proteins reveals that hexokinase-2 is inhibited by arsenic. Proc Natl Acad Sci USA 112(49):15084-9. [PubMed]
Hu J et al. (2015) Systematic Prediction of Scaffold Proteins Reveals New Design Principles in Scaffold-Mediated Signal Transduction. Comput Biol 112(9): e1004508. [PubMed]
Cox E et al. (2015) Identification of SUMO E3 ligase-specific substrates using the HuProt human proteome microarray. Methods Mol Biol 1295:455-63. [PubMed]
Hu C et al. (2015) Autoantibody Profiling on Human Proteome Microarray for Biomarker Discovery in Cerebrospinal Fluid and Sera of Neuropsychiatric Lupus. PLoS One 10:e0126643. [PubMed] [Full text]
Liu S et al. (2014) Characterization of monoclonal antibody's binding kinetics using oblique-incidence reflectivity difference approach. MAbs 7:110-119. [PubMed] [Full text]
Jung JG et al. (2014) Notch3 interactome analysis identified WWP2 as a negative regulator of Notch3 signaling in ovarian cancer. PLos Genet 10:e1004751. [PubMed]
Fan Q et al. (2014) Identification of proteins that interact with alpha A-crystallin using a human proteome microarray. Mol Vis 20:117–124. [PubMed]
Deng RP et al. (2014) Global Identification of O-GlcNAc Transferase (OGT) Interactors by a Human Proteome Microarray and the Construction of an OGT Interactome. Proteomics 14(9): 1020-30. doi: 10.1002/pmic.201300144. Epub 2014 Mar 25.0. [PubMed]
Ma TM et al. (2014) Serine Racemase Regulated by Binding to Stargazin and PSD-95: Potential NMDA-AMPA Glutamate Neurotransmission Cross-talk. J Biol Chem pii: jbc. M114.571604 [Epub ahead of print]; First Published on August 27, 2014. [PubMed]
Barry G et al. (2013) The long non-coding RNA Gomafu is acutely regulated in response to neuronal activation and involved in schizophrenia-associated alternative splicing. Molec Psychiatry Apr 30. doi: 10.1038/mp.2013.45. ePub ahead of print. [PubMed]
Lee YI et al. (2013) Protein microarray characterization of the S-nitrosoproteome. Mol Cell Proteomics 13:63-72. [PubMed]
Chen Y et al. (2013) Bcl2-associated Athanogene 3 Interactome Analysis Reveals a New Role in Modulating Proteasome Activity. Mol Cell Proteomics 12(10):2804-19. [PubMed]
Donnelly CJ et al. (2013) RNA toxicity from the ALS/FTD C9ORF72 expansion is mitigated by antisense intervention. Neuron 80(2):415-28. [PubMed]
Fan B et al. (2013) A human proteome microarray identifies that the heterogeneous nuclear ribonucleoprotein K (hnRNP K) recognizes the 5’ terminal sequence of the hepatitis C virus RNA. Mol Cell Proteomics 13(1):84-92. [PubMed]
Tarrant MK et al. (2012) Regulation of CK2 by phosphorylation and O-GlcNAcylation revealed by semisynthesis. Nat Chem Biol 8(3):262-9. [PubMed]
Jeong JS et al. (2012) Rapid identification of monospecific monoclonal antibodies using a human proteome microarray. Mol Cell Proteomics 11(6):O111.016253. [PubMed]
Huang Y et al. (2012). Global tumor protein p53/p63 interactome: making a case for cisplatin chemoresistance. Cell Cycle 11(12):2367-79. [PubMed]
Hu CJ et al. (2012). Identification of new autoantigens for primary biliary cirrhosis using human proteome microarrays. Mol Cell Proteomics 11(9):669-80. [PubMed]