The HuProt™ Human Proteome Microarray contains the world's largest number of unique, individually-purified human proteins. That’s thousands more than the not-so-close second.
The comprehensive content of the HuProt™ microarray allows thousands of interactions to be profiled in high-throughput.
in 16,793 genes, covering ~81% of the canonical proteome – as defined by the Human Protein Atlas. (www.proteinatlas.org/about/licence)
Below: CDI HuProt™ microarray comprising GST fusion proteins probed with anti-GST antibody.
The HuProt™ human proteome microarray v4.0 contains >21,000 unique, newly resequenced and individually purified human proteins encompassing 16,793 genes (~81% of the canonical proteome – as defined by the Human Protein Atlas [www.proteinatlas.org/about/licence]) and 124 unique mouse gene symbols. Recombinant proteins are expressed in yeast (S. cerevisiae), purified and printed on glass slides in duplicate, along with control proteins.
Search the content of the HuProt Microarray. Click HERE to access searchable files in our "Resources" page.
State-of-the-Art Manufacturing Technology
After expression, the N-terminal GST- and His6-tagged proteins are purified and printed on glass slides in duplicate, along with control proteins (GST, BSA, Histones, IgG, etc.) and new controls that allow for the use of linear regression analysis, radio-labeled sample analysis and the use of different analytical software.
Slides are bar-coded for tracking/archiving. Each microarray batch is routinely evaluated by GST immunoblotting (98% of all proteins authenticate to their respective molecular weights).
Flexibility is built-in. The HuProt™ human proteome microarray is available on two types of glass surfaces: PATH™ and SuperEpoxy2™.
Focused arrays can be designed out of biomarkers identified within the HuProt™ discovery phase. Efficient (up to 2x7) slide formats can be configured for a cost-effective and rapid approach to the validation phase. VIDEO DEMO! LEARN MORE
The HuProt human proteome microarray can be used for many applications.
in more than 16,793 genes (~81% of the canonical proteome – as defined by the Human Protein Atlas [www.proteinatlas.org/about/licence])
CDI recommends the GenePix platform for data analyis once the experimental procedures are completed. The HuProt human proteome microarrays include .gal (GenePix Array List) files for used for data analyis once the experimental procedures are completed. All GenePix scanners include one license of GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.
For the analysis of data generated using the HuProt microarray, click HERE to access the GenePix .gal files in our "Resources" page
CDI supplies a comprehensive USER GUIDE with the HuProt Proteome Microarray. It includes storage and handling tips, material requirements and detailed procedures for eight assays. A lot-specific Certificates of Analysis (COAs) with quality assurance data is also included.
To place an order for the HuProt Human Proteome Microarray, simply give us a call at 1-844-539-6296 and request:
- Catalog # CDI-HP-003.1
- Price $1,120
10-15 --- 15% discount
16-30 --- 20% discount
31+ ----- 25% discount
Further discounts on 100+ quantities are available. Please call us at 1-844-539-6296.
Custom microarray configurations are available.
Our techical representatives are standing by to assist you.
Using the HuProt Microarray, CDI can do the work for you so you can concentrate on your research. We offer expertise in high-throughput design and provide detailed data analysis. Our techical representatives are standing by to assist you.
Click HERE for more information regarding CDI Custom Discovery Services.
Developed an antibody? The specificity will be critical to the success of your research. HighSpec® Antibody Validation is offered to assess cross-reactivity to thousands of human proteins in the array – BEFORE you publish. Click HERE for more information on HighSpec.
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