The HuProt™ Human Proteome Microarray contains the world's largest number of unique, individually-purified human proteins. That’s thousands more than the not-so-close second.

The comprehensive content of the HuProt™ microarray allows thousands of interactions to be profiled in high-throughput.

in more than 15,000 genes, covering ~75% of the proteome.

Below: CDI HuProt™ microarray comprising GST fusion proteins probed with anti-GST antibody.

HuProt™ human proteome microarray v3.0

The HuProt™ human proteome microarray v3.0 contains >19,000 unique, newly resequenced and individually purified human proteins encompassing >15,000 genes (~75% of the human proteome; see figure, right) and 124 unique mouse gene symbols. Recombinant proteins are expressed in yeast (S. cerevisiae), purified and printed on glass slides in duplicate, along with control proteins.

categories of proteins in the HuProt human protein microarray

Search the content of the HuProt Microarray. Click HERE to access searchable files in our "Resources" page.

NEW in v3.0 - State-of-the-Art Manufacturing Technology

piezoelectric microarray spottingAfter expression, the N-terminal GST- and His6-tagged proteins are purified and printed on glass slides in duplicate, along with control proteins (GST, BSA, Histones, IgG, etc.).

Slides are bar-coded for tracking/archiving. Each microarray batch is routinely evaluated by GST immunoblotting (98% of all proteins authenticate to their respective molecular weights).

spot morphology. distribution, and accuracy are greatly improved by utilizing piezoelectric microarray printing

The HuProt microarray is available on a variety* of glass surfaces, i.e. FAST™, FullMoon™, SuperEpoxy™, SuperAldehyde™, SuperNHS™, Ni-NTA, PATH, etc,

AND... Focused arrays can be designed out of biomarkers identified within the HuProt discovery phase. Efficient (up to 2x7) slide formats can be configured for a cost-effective and rapid approach to the validation phase.

Surfaces available for the HuProt microarray

*CDI’s standard substrate for printing is SuperEpoxy2. If other substrates are requested there is a two week waiting period.

The HuProt human proteome microarray can be used for many applications.

in more than 15,000 genes, covering ~75% of the proteome.

illustration of the protein library on HuProt

CDI recommends the GenePix platform for data analyis once the experimental procedures are completed. The HuProt human proteome microarrays include .gal (GenePix Array List) files for used for data analyis once the experimental procedures are completed. All GenePix scanners include one license of GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images.

microarray data analysis

For the analysis of data generated using the HuProt microarray, click HERE to access the GenePix .gal files in our "Resources" page

CDI supplies a comprehensive USER GUIDE with the HuProt Proteome Microarray. It includes storage and handling tips, material requirements and detailed procedures for eight assays. A lot-specific Certificates of Analysis (COAs) with quality assurance data is also included.

HuProt user guide

To place an order for the HuProt Human Proteome Microarray, simply give us a call at 787-806-4006 and request:

  • Catalog # CDI-HP-002
  • Price $1035.00
image description

Quantity discounts are available.
Please inquire.

Custom microarray configurations are available.
Our techical representatives are standing by to assist you.

Using the HuProt Microarray, CDI can do the work for you so you can concentrate on your research. We offer expertise in high-throughput design and provide detailed data analysis.

Developed an antibody? The specificity will be critical to the success of your research. HighSpec® Antibody Validation is offered to assess cross-reactivity to thousands of human proteins in the array – BEFORE you publish.

Our techical representatives are standing by to assist you.

Click HERE for more information regarding
CDI Custom Discovery Services.

antibody validation

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