ChIP-Seq
Technical and Sales Support

CDI scientists are available to answer any questions that you may have regarding our products and services:

Tel 787-806-4006 Tel 844-539-6296 (TOLL FREE US/Canada) [email protected] Sales inquiries

The development of the these antibodies was supported by the National Institutes of Health Protein Capture Reagent Program under award U54HG06434 to CDI Laboratories and Johns Hopkins University.

Microarray-powered

ChIP-Seq mMAbs

Specifity-Validated, Application-Tested &
Affinity-Purified Transcription Factor Antibodies

CDI launches its line of monospecific, affinity-purified monoclonal antibodies to several human transcription factors. This initial offering is highly suited for ChIP sequencing - the analysis of protein-DNA interactions.

CDI utilizes its HuProt™ Human Proteome Microarray for validating the specificity of the final product. These antibodies have been screened for cross-reactivity against thousands of human proteins (~75% of the human proteome).

Be Specific. If you’re grant-writing, publishing or manufacturing...
make CDI mMAbs part of your discovery and development success.

Current CDI transcription factor mMAb offering
(click Cat # for lot specific datasheet):

Target Clone ID Applications Format Cat. # Status Price
ARID3A R21.1.1E9 IP, ChIP-Seq 100 µg m13-034 in stock $349
BATF R451.2.1E10 IP, ChIP-Seq 100 µg m14-108 in stock $349
DPF1 R1172.1.1A9 IP, ChIP-Seq 100 µg m15-076 in stock $349
DPF1 R1172.1.1B6 IP, WB, ChIP-Seq 100 µg m15-066 in stock $349
ETV6 R1092.1.1A9 IP, WB, ChIP-Seq 100 µg m15-030 in stock $349
ETV6 R1092.1.1A7 IP, WB, ChIP-Seq 100 µg m15-248 in stock $349
HES1 R159.4.1B11 IP, ChIP-Seq 100 µg m13-003 in stock $349
KLF10 R846.1.1D3 IP, WB, ChIP-Seq 100 µg m14-355 in stock $349
L3MBTL4 R160.1.1C8 IP, WB, ChIP-Seq 100 µg m13-094 in stock $349
NFATC3 R1106.1.2A12 IP, ChIP-Seq 100 µg m15-047 in stock $349
NRF1 R157.1.3D4 IP, WB, ChIP-Seq 100 µg m13-005 in stock $349
RUVBL1 JH39.2.1A1 IP, WB, ChIP-Seq 100 µg m13-077 in stock $349
SMAD4 R516.2.1D12 IP, ChIP-Seq 100 µg m14-150 in stock $349
SSBP4 JH97.1.3B5 IP, ChIP-Seq 100 µg m14-286 in stock $349
TSC22D4 R160.2.2A2 IP, ChIP-Seq 100 µg m13-016 in stock $349
USF2 R1156.1.1A7 IP, ChIP-Seq 100 µg m15-084 in stock $349
WDHD1 R1251.1.1A5 IP, WB, ChIP-Seq 100 µg m15-092 in stock $349
WDHD1 R1251.1.1B10 IP, WB, ChIP-Seq 100 µg m15-104 in stock $349
ZBTB2 R6.1.3.46F2 IP, ChIP-Seq 100 µg m13-048 in stock $349
ZMYM3 R259.2.2C3 IP, WB, ChIP-Seq 100 µg m15-005 in stock $349
ZMYM3 JH39.2.2B9 IP, WB, ChIP-Seq 100 µg m15-026 in stock $349
ZNF18 R177.1.2D2 IP, WB, ChIP-Seq 100 µg m15-035 in stock $349
ZNF410 R585.2.2C9 IP, ChIP-Seq 100 µg m14-038 in stock $349
ZNF597 R917.1.1C12 IP, ChIP-Seq 100 µg m15-058 in stock $349
ZNF639 R270.2.2B2 IP, WB, ChIP-Seq 100 µg m13-088 in stock $349
ZNF639 R270.2.1E7 IP, ChIP-Seq 100 µg m13-049 in stock $349
ZNF701 JR1015.1.1C5 IP, WB, ChIP-Seq 100 µg m14-316 in stock $349
ZNF75A R1014.1.1B6 IP, WB, ChIP-Seq 100 µg m15-003 in stock $349
ZXDC JH98.1.2B5 IP, WB, ChIP-Seq 100 µg m14-284 in stock $349
Quality Assurance - HuProt TM Microarray Specificity
(Representative Data)
antibody validation and quality assurance

Microarray Analysis: Anti-Human SMAD4 (clone R516.2.1D12) specificity was evaluated using CDI HuProt™ Human Proteome Microarray and subsequently analyzed with GenePix Pro Image Acquisition and Analysis Software, the benchmark tool for the acquisition and analysis of microarray images. The top 3 “hits” were identified by cross- reference to the array map which stores the exact location of each protein. The expected target, SMAD4, is ranked #1 and is considered monospecific. Signals ranked beyond #1 are statistically insignificant and not displayed.

antibody specificity data

Statistical Evaluation: GenePix data points (from above) were analyzed in terms of signal strength and ranked accordingly. The value shown for rank #1 and rank #2 indicate that Anti-Human SMAD4 (clone R516.2.1D12) is monospecfic. Rankings 2 through the remainder of the array are essentially equivalent to non-specific background signal.

A-Score (Affinity Score) is the normalized signal intensity of spots on the HuProt Array. S-Score (Specificity Score) S = A(Rank1) - A2(Rank2). A- and S-scores greater than 3 standard deviations over the next listed target are deemed statistically significant and indicate highly specific antibodies with high affinity. In the data above, the difference between Rank 1 and Rank 2 is 87.82. (standard deviations). This indicates that the antibody is monospecfic for SMAD4.

Validated Applications - ChIP-Seq (Representative Data)
ChIP-Seq analysis

ChIP-Seq Analysis: The ChIP was performed with chromatin from 10 million HepG2 cells and 3 μg of Anti-SMAD4 (cloneID #R516.2.1D12) antibody. The ChIP DNA was sequenced on an Illumina HiSeq platform and read counts were calculated at consecutive 100 bp bins across the human genome hg19. Normalized read-count levels for ChIP-seq of SMAD4 (R516.2.1D12_ChIP) and control (R516.2.1D12_IN) around the SKIL and JHDM1D loci are displayed in the CisGenome browser.